#Apply compensation matrix flowjo software#
#Apply compensation matrix flowjo series#

Derived ParametersįlowJo lets you define new parameters for samples. FlowJo adds compensated parameters to the multi-stained samples so you can view and analyze compensated data. You can then apply this compensation matrix to multi–stained samples. The interface for computing the compensation matrix is based on a collection of singly-stained control samples. Spectral overlap of stains is dealt with in the compensation tool in FlowJo. All models can be copied to other populations or samples, with the same easy drag and drop interface used to propagate other gates or analyses. Cell Cycle AnalysisįlowJo has a Cell Cycle Platform that uses multiple models to fit the data, constrains the fitting parameters, and automatically calculates the percentage of cells in the G1, S, or G2 phases. Rather, the focus is on analyzing groups of samples, experiments, or even multiple experiments at once. In this way, FlowJo is a program that doesn’t just analyze individual data samples. In general, batch operations are performed on all samples in the current group (therefore, you will want to create sample groups that will serve to help you generate batch outputs). Batch Operationsīatch operations (repetitive analyses performed on multiple samples) can be used in creating layouts and tables in FlowJo. Backgating analysis shows graphs of each gating operation with the final subpopulation highlighted in the graph of each stage. Once your analysis contains subpopulations produced by gating within gates, it can be useful to see the source of a given subpopulation rendered graphically.

#Apply compensation matrix flowjo software#

Good template design saves a huge amount of software analysis time and standardizes reporting. Next week, when you do the same experiment with different patients, most of the work is already done for you! You simply have to import the new samples into the template workspace. If you do similar experiments with different patients, you can then save a workspace as a template containing all the gates, statistics, tables, and layouts.

Generate a table in which you can create particular statistics from all samples.

Generate a graphical layout in which you can display particular graphs from all samples.

Check gates on the samples in the group: modifications to accommodate sample to sample variation are easily made.

Apply the appropriate analyses to all the samples in a group.

Analyze a single sample in detail, creating gates, statistics, etc.

Create Groups for related samples, gates, etc.

#Apply compensation matrix flowjo series#

You will find that your mode of operating FlowJo will probably be very similar to the following series of steps: FlowJo integrates remote data hosting services (LIMS) and supports “cloud” deployment for excellent scaling of performance in a demanding, high-throughput environment.

FlowJo’s sophisticated tools allow for generation of statistics, graphs, tables, web pages, and even movies. In addition to gating and statistics, FlowJo has specialized analysis platforms for assays involving DNA/Cell Cycle, Kinetics, and Proliferation, among others. FlowJo is an integrated environment for viewing and analyzing flow cytometric data.įlowJo’s strength is in analyzing whole experiments encompassing many related samples.